Surface-Enhanced Raman Spectroscopy

January 13. 2016 | ISBN-10: 3319239902 | 221 Pages | pdf | 6.3 MB

Covers applications of surface-enhanced Raman scattering technique in the life sciences
Presents the instrumentations of SERS for bioanalytical studies and sensing
The didactic, comprehensible approach and the many illustrative figures makes the book accessible to students as well

About this book

This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.

Number of Illustrations and Tables
51 illus., 27 in colour
Topics
Biophysics and Biological Physics
Biological Techniques
Spectroscopy and Microscopy
Measurement Science, Instrumentation
Medical and Radiation Physics

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